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Anti-PCNA Antibody (aa1-261, clone K1A3) LS-C93776

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PCNA Antibodies

Anti-PCNA Antibody (Internal) IHC-plus™ LS-B402
Rabbit Polyclonal to Human PCNA
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody (C-Terminus) IHC-plus™ LS-B2826
Rabbit Polyclonal to Human PCNA
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody IHC-plus™ LS-B4230
Mouse Monoclonal (IgG2a) to Rat PCNA
Rat, Human, Insect, S. pombe, Vertebrate
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone K1A3] (IgG3) to Human PCNA
Human
Western blot, ELISA
Unconjugated

Details

Human PCNA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG3 Monoclonal [K1A3]
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1:1000 - 1:3000)
  • ELISA

  • IHC - Paraffin

Specificity and Use

PCNA antibody was raised against recombinant human PCNA (1-261aa) purified from E. coli.
aa1-261
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:3000.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PCNA

P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Western blot

Western blot
Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PCNA (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/7/2016

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