Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Human, Chicken (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:500)
IHC - Frozen
Immunofluorescence (1:50 - 1:250)
Western blot (1:50 - 1:250)
Immunoprecipitation (1:50 - 1:250)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
PAX3 antibody was raised against his6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains.
Recognizes the ~53kD Pax3 protein in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1:50-1:250. Western Blot: 1:50-1:250. Immunoprecipitation: 1:50-1:250. Immunohistochemistry (Frozen and paraffin): 1:50-1:250. Use with formalin-fixed, paraffin-embedded mouse tissue sections, requires heat induced epitope retrieval (HIER) using citrate buffer, pH 6.0 for 10 minutes.
Supplied a liquid, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma.