(tested or 100% immunogen sequence identity)
Human, Chicken (at least 90% immunogen sequence identity)
- IHC - Paraffin (1:100 - 1:500)
- IHC - Frozen
- Immunofluorescence (1:50 - 1:250)
- Western blot (1:50 - 1:250)
- Immunoprecipitation (1:50 - 1:250)
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PAX3 antibody was raised against his6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains.
Recognizes the ~53kD Pax3 protein in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1:50-1:250. Western Blot: 1:50-1:250. Immunoprecipitation: 1:50-1:250. Immunohistochemistry (Frozen and paraffin): 1:50-1:250. Use with formalin-fixed, paraffin-embedded mouse tissue sections, requires heat induced epitope retrieval (HIER) using citrate buffer, pH 6.0 for 10 minutes.
Supplied a liquid, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.