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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAX3 Antibody (N‑Terminus) LS‑C9504 Best Seller
PAX3 antibody LS-C9504 is an unconjugated rabbit polyclonal antibody to mouse PAX3. Validated for IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C9504-100
100 µl
Unavailable

Popular PAX3 Products

Anti-PAX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1554 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
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Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
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Product Description

PAX3 antibody LS-C9504 is an unconjugated rabbit polyclonal antibody to mouse PAX3. Validated for IF, IHC, IP and WB.
About PAX3
PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. NM_000438 NP_000429.2

PAX3 Antibody, CDHS Antibody, Paired domain gene 3 Antibody, Paired box protein Pax-3 Antibody, Paired domain gene HuP2 Antibody, Waardenburg syndrome 1 Antibody, WS1 Antibody, HUP2 Antibody, Paired box 3 Antibody, Paired box homeotic gene 3 Antibody, WS3 Antibody


Specifications

Target
Mouse PAX3
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • IHC - Frozen
  • Immunofluorescence (1:50 - 1:250)
  • Western blot (1:50 - 1:250)
  • Immunoprecipitation (1:50 - 1:250)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PAX3 antibody was raised against his6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains.
Epitope
N-Terminus
Specificity
Recognizes the ~53kD Pax3 protein in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1:50-1:250. Western Blot: 1:50-1:250. Immunoprecipitation: 1:50-1:250. Immunohistochemistry (Frozen and paraffin): 1:50-1:250. Use with formalin-fixed, paraffin-embedded mouse tissue sections, requires heat induced epitope retrieval (HIER) using citrate buffer, pH 6.0 for 10 minutes.
Presentation
Supplied a liquid, 0.1% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/19/2018

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