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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAX3 Antibody (N‑Terminus) LS‑C9504
PAX3 antibody LS-C9504 is an unconjugated rabbit polyclonal antibody to mouse PAX3. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C9504-100
100 µl
$605
Description
PAX3 antibody LS-C9504 is an unconjugated rabbit polyclonal antibody to mouse PAX3. Validated for IF, IHC, IP and WB.
Target
Mouse PAX3
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • IHC - Frozen
  • Immunofluorescence (1:50 - 1:250)
  • Western blot (1:50 - 1:250)
  • Immunoprecipitation (1:50 - 1:250)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PAX3 antibody was raised against his6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains.
Epitope
N-Terminus
Specificity
Recognizes the ~53kD Pax3 protein in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1:50-1:250. Western Blot: 1:50-1:250. Immunoprecipitation: 1:50-1:250. Immunohistochemistry (Frozen and paraffin): 1:50-1:250. Use with formalin-fixed, paraffin-embedded mouse tissue sections, requires heat induced epitope retrieval (HIER) using citrate buffer, pH 6.0 for 10 minutes.
Presentation
Supplied a liquid, 0.1% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About PAX3

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Requested From: United States
Date Requested: 12/15/2018
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