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Catalog Number Size Price
LS-C47555-100 100 µg (0.5 mg/ml) $503 
PAX3 Antibody - PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.
PAX3 Antibody - PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
PAX3 Antibody - PAX3 antibody (3 ug/ml) staining of paraffin embedded Human Esophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
PAX3 Antibody - Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
PAX3 Antibody - PAX3 antibody (0.3µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PAX3 Antibody - PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.
PAX3 Antibody - PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
PAX3 Antibody - PAX3 antibody (3 ug/ml) staining of paraffin embedded Human Esophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
PAX3 Antibody - Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
PAX3 Antibody - PAX3 antibody (0.3µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Polyclonal Goat anti‑Human PAX3 Antibody (aa2‑12, IHC, WB) LS‑C47555

Polyclonal Goat anti‑Human PAX3 Antibody (aa2‑12, IHC, WB) LS‑C47555

Note: This antibody replaces LS-C93084
Antibody:
PAX3 Goat anti-Human Polyclonal (aa2-12) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C47555-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PAX3 Goat anti-Human Polyclonal (aa2-12) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
PAX3 antibody LS-C47555 is an unconjugated goat polyclonal antibody to PAX3 (aa2-12) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB.
Target
Human PAX3
Synonyms
PAX3 | CDHS | Paired domain gene 3 | Paired box protein Pax-3 | Paired domain gene HuP2 | Waardenburg syndrome 1 | WS1 | HUP2 | Paired box 3 | Paired box homeotic gene 3 | WS3
Host
Goat
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence TTLAGAVPRMM-C, from the N-Terminus of protein sequence according to NP_852122.1NP_000429.2NP_039230.1NP_852123.1NP_852124.1NP_852126.1NP_852125.1NP_001120838.1.
Epitope
aa2-12
Specificity
Human PAX3. This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2; NP_039230.1; NP_852122.1;.1 NP_852123.1; NP_852124.1; NP_852125.1 and NP_852126.1).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3 ug/ml. Western blot: Preliminary experiments gave an approx 30-35 kDa band in Human Kidney lysates at 1u ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2; NP_039230.1; NP_852122;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PAX3

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Requested From: United States
Date Requested: 4/18/2024