Target
Human PAX3
Synonyms
PAX3 | CDHS | Paired domain gene 3 | Paired box protein Pax-3 | Paired domain gene HuP2 | Waardenburg syndrome 1 | WS1 | HUP2 | Paired box 3 | Paired box homeotic gene 3 | WS3
Reactivity
Human, Monkey, Dog, Guinea pig, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-STVHQSTIPSNPD, from the internal region of the protein sequence according to NP_852122.1NP_000429.2NP_039230.1NP_852123.1NP_852124.1NP_852126.1NP_852125.1NP_001120838.1.
Specificity
Human PAX3. This antibody is expected to recognize isoforms PAX3 (NP_852122.1), PAX3d (NP_852123.1), PAX3e (NP_852124.1), PAX3g (NP_852126.1), PAX3h (NP_852125.1), and PAX3i (NP_001120838.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 55kD band observed in Human Brain (Frontal Cortex) and Mouse and Rat Brain lysates (calculated MW of 53.0kD according to Human NP_852122.1 and 52.9kD according to Mouse NP_032807.3). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee