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PARP1 antibody LS-C17215 is an unconjugated rabbit polyclonal antibody to human PARP1. Validated for ELISA, Flow, ICC, IF, IHC and WB.
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Species:
Human, Mouse, Rat, Hamster, Xenopus, Drosophila
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Dog, Hamster, Rabbit
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Product Description
PARP1 Antibody (C-Terminus) for ELISA, Flow, ICC, IF/Immunofluorescence, IHC, WB/Western LS-C17215
Specifications
Target
Human PARP1
Synonyms
PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (1:50)
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Flow Cytometry (1:25)
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Immunogen
PARP1 antibody was raised against synthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in human PARP.
Epitope
C-Terminus
Specificity
Detects endogenous levels of the large fragment (89kD) of human PARP produced by caspase cleavage. Does not react with full length PARP.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (IF): 1:200. Flow Cytometry: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PARP1
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR.
P09874
NM_001618
NP_001609.2
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Requested From: United States
Date Requested: 10/15/2018
Date Requested: 10/15/2018
