PARP1 antibody LS-C17215 is an unconjugated rabbit polyclonal antibody to human PARP1. Validated for ELISA, Flow, ICC, IF, IHC and WB.
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (1:50)
- Western blot (1:1000)
- Flow Cytometry (1:25)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
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PARP1 antibody was raised against synthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in human PARP.
Detects endogenous levels of the large fragment (89kD) of human PARP produced by caspase cleavage. Does not react with full length PARP.
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (IF): 1:200. Flow Cytometry: 1:25.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.