Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Guinea pig (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:1000)
Specificity and Use
PAK1 / PAK2 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser199/204 of human PAK1.
Detects endogenous levels of Ser199/204 phosphorylated human PAK1 or Ser192/197 phosphorylated human PAK2. It may also detect Ser200/205 phosphorylated PAK3, however it does not cross-react with phosphorylated PAK4. Species cross-reactivity: Guinea pig. Predicted to cross-react with mouse and rat based on sequence homology.
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.