Human, Mouse, Rat, Guinea pig
(tested or 100% immunogen sequence identity)
Protein A purified
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
Recognizes Ser144 phosphorylated PAK1 and Ser141 phosphorylated PAK2. This antibody also recognizes Ser139 phosphorylated PAK3 but does not cross-react with phosphorylated PAK4-6. The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth. Species cross-reactivity: Human and guinea pig, predicted to react with rat and mouse due to 100% sequence homology.
Suitable for use in Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.