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Anti-P2RX1 / P2X1 Antibody (aa364-372) LS-C38011

Ordering

Wt. Vol. Conc. Price
- 50 µl - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular P2RX1 / P2X1 Antibodies

Anti-P2RX1 / P2X1 Antibody (aa345-372) LS-C163313
Rabbit Polyclonal to Human P2RX1 / P2X1
Human
Western blot
Unconjugated
Western blot Image
Anti-P2RX1 / P2X1 Antibody (aa344-372, APC) LS-C265369
Rabbit Polyclonal (IgG) to Human P2RX1 / P2X1
Human
Western blot, ELISA
APC Conjugated
Anti-P2RX1 / P2X1 Antibody (aa344-372, Azide-free, HRP) LS-C265375
Rabbit Polyclonal (IgG) to Human P2RX1 / P2X1
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat P2RX1 / P2X1
Rat
IHC, ICC, Immunofluorescence
Unconjugated

Details

Rat P2RX1 / P2X1
Rabbit
Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC (1:1000)
  • ICC (1:1000)
  • Immunofluorescence

Specificity and Use

P2RX1 / P2X1 antibody was raised against synthetic peptide corresponding to residues 364-372 of the rat P2X1.
aa364-372
Species Reactivity: Reacts with Rat.
Suitable for use in Immunohistochemistry, Immunocytochemistry. Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: 1:1000. P2X1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Note: Sodium azide interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.

Packaging

0.05% sodium azide.
For research use only.

About P2RX1 / P2X1

P51575 NM_002558 NP_002549.1

P2RX1 Antibody, ATP receptor Antibody, p2X receptor 1 Antibody, p2X1 Antibody, p2X1 purinoceptor Antibody, p2X1 receptor Antibody, Purinergic receptor Antibody, p2RX1 Antibody, p2X purinoceptor 1 Antibody, p2X receptor, subunit 1 Antibody

P2RX1 / P2X1 belongs to the P2X family of G-protein-coupled receptors. These proteins can form homo-and heterotimers and function as ATP-gated ion channels and mediate rapid and selective permeability to cations. This protein is primarily localized to smooth muscle where binds ATP and mediates synaptic transmission between neurons and from neurons to smooth muscle and may being responsible for sympathetic vasoconstriction in small arteries, arterioles and vas deferens.

Requested From: United States
Date Requested: 12/4/2016

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