Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human NT5E / eNT / CD73
IHC, Western blot, Flow Cytometry, ELISA
Mouse Monoclonal [clone AD2] (IgG1,k) to Human NT5E / eNT / CD73
Human NT5E / eNT / CD73
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AD2]
Specificity and Use
This AD2 antibody has been pre-titrated and tested by flow cytometric analysis on activated normal human peripheral blood cells. This can be used at 5 ul (0.125 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
NT5E / eNT / CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Surface staining of 3-day CD3/CD28-activated CD4+CD25+ normal human peripheral blood cells with APC Mouse IgG1, K isotype control (left) or APC anti-human CD73 (right) followed by intracellular staining with PE anti-human Foxp3 (PCH101). Cells in the CD4+CD25+ gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.