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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3428-200 | 200 µl (2 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human NT5E / eNT / CD73 Antibody (aa520‑550, IHC, WB) LS‑B3428
IHC‑plus™ Polyclonal Rabbit anti‑Human NT5E / eNT / CD73 Antibody (aa520‑550, IHC, WB) LS‑B3428
Note: This antibody replaces LS-C97671
Antibody:
NT5E / eNT / CD73 Rabbit anti-Human Polyclonal (aa520-550) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NT5E / eNT / CD73 Rabbit anti-Human Polyclonal (aa520-550) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
CD73 antibody LS-B3428 is an unconjugated rabbit polyclonal antibody to CD73 (NT5E / eNT) (aa520-550) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human NT5E / eNT / CD73
Synonyms
NT5E | 5-nucleotidase, ecto (CD73) | 5'-nucleotidase | 5 nucleotidase (CD73) | 5-NT | 5-nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 | CD73 antigen | E5NT | Ecto-5-nucleotidase | ENT | EN | NT | Purine 5-Prime-Nucleotidase | NT5 | NTE
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa520-550
Specificity
This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3428 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3428 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NT5E / eNT / CD73
Validation
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NT5E / eNT / CD73 Antibody (aa520-550) for IHC, WB/Western LS-B3428 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Hypoxia-inducible factor-1-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor. Hart ML, Grenz A, Gorzolla IC, Schittenhelm J, Dalton JH, Eltzschig HK. Journal of immunology (Baltimore, Md. : 1950). 2011 186:4367-74.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
Western blot, Flow Cytometry, ELISA
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/20/2024
Date Requested: 4/20/2024
