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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NT5E / eNT / CD73 Antibody (clone 4C4‑2B5) LS‑C197804
CD73 antibody LS-C197804 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT). Validated for ELISA and WB.
Catalog
Size
Price
LS-C197804-100
100 µg
Unavailable

Popular NT5E / eNT / CD73 Products

Antibody
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA
Anti-NT5E / CD73 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2943 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3428 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-NT5E / CD73 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3897 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Anti-CD73 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8284 dilution 5-10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CD73 antibody LS-C197804 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT). Validated for ELISA and WB.
About NT5E / eNT / CD73
NT5E / eNT / CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. P21589 NM_002526 NP_002517.1

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Specifications

Target
Human NT5E / eNT / CD73
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [4C4-2B5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
NT5E (AAH15940.1, aa28-264 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human NT5E / CD73
Usage
Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.Lane 1: NT5E transfected lysate(30.36 KDa).Lane 2: Non-transfected lysate.


Requested From: 
Date Requested: 6/18/2018

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