Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Western blot (0.1 - 0.2 µg/ml)
ELISA (0.5 - 1 µg/ml)
Specificity and Use
NT5E / eNT / CD73 antibody was raised against cHO cell-derived rmCD73. (aa 29 - 549).
Recognizes mouse CD73.
Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Direct ELISA: 0.5-1.0 ug/ml. Detection limit for rmCD73 is. ~0.3 ng/well. Western Blot: 0.1-0.2 ug/ml. Detection limit for rmCD73 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry (Murine splenocytes): 25 ug/ml. Add 100 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul.
Lyophilized from PBS, 5% trehalose.
500 µl PBS, 40-50% glycerol
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid frequent freeze thaws.
NT5E / eNT / CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.