Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 7D5] (IgG2a) to Rat NRG1 / Heregulin / Neuregulin
Rat, Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Rat NRG1 / Heregulin / Neuregulin
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [7D5]
Also available conjugated with Biotin.
Protein A purified
IHC - Paraffin (1:25 - 1:50)
Western blot (1:25 - 1:50) & (1 - 2 µg/ml)
Immunoprecipitation (1:25 - 1:50) & (1 - 2 µg/ml)
(applications tested for the base form of this product only)
Specificity and Use
NRG1 / Heregulin / Neuregulin antibody was raised against recombinant extracellular domain of rat NDF protein.
When used with formalin-fixed paraffin-embedded tissues for IHC this antibody requires that the tissues be boiled in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
10mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide
Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF).
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 1/19/2017