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Anti-NR1I3 / CAR Antibody (aa180-229) IHC-plus™ LS-B3559

Note: This antibody replaces LS-C102177

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NR1I3 / CAR Antibodies

Anti-NR1I3 / CAR Antibody (C-Terminus) LS-C30862
Rabbit Polyclonal (IgG) to Mouse NR1I3 / CAR
Mouse
IHC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NR1I3 / CAR Antibody (N-Terminus) IHC-plus™ LS-B2628
Rabbit Polyclonal to Human NR1I3 / CAR
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NR1I3 / CAR Antibody (aa180-229) IHC-plus™ LS-B3559
Rabbit Polyclonal (IgG) to Human NR1I3 / CAR
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NR1I3 / CAR
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NR1I3 / CAR
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NR1I3 / CAR antibody was raised against synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%); Zebrafish (85%); Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%).
LS-E16655 - Lyophilized - 100 µg - $145.00
aa180-229
Human NR1I3
Immunohistochemistry: LS-B3559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3559 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NR1I3 / CAR

Q14994 NM_005122 NP_005113.1

NR1I3 Antibody, Car-beta Antibody, CAR1 Antibody, Constitutive active receptor Antibody, Constitutive active response Antibody, Orphan nuclear receptor MB67 Antibody, MB67 Antibody, CAR Antibody

NR1I3 / CAR is a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members.

Immunohistochemistry

Immunohistochemistry
Anti-NR1I3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3559 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NR1I3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3559 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
NR1I3 antibody LS-B3559 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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