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Anti-NR1H3 / LXR Alpha Antibody (C-Terminus) IHC-plus™ LS-B3526

Note: This antibody replaces LS-C80223

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NR1H3 / LXR Alpha Antibodies

Anti-NR1H3 / LXR Alpha Antibody (aa164-447) LS-C9359
Mouse Monoclonal (IgG2a) to Human NR1H3 / LXR Alpha
Human, Rat
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-NR1H3 / LXR Alpha Antibody (C-Terminus) IHC-plus™ LS-B3526
Rabbit Polyclonal (IgG) to Human NR1H3 / LXR Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NR1H3 / LXR Alpha Antibody (C-Terminus, Magnetic beads) LS-C172076
Rabbit Polyclonal to Human NR1H3 / LXR Alpha
Human, Rat
Western blot, Immunoprecipitation
Magnetic beads Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human NR1H3 / LXR Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NR1H3 / LXR Alpha
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

NR1H3 / LXR Alpha antibody was raised against synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%).
LS-E13920 - Lyophilized - 100 µg - $145.00
C-Terminus
Human NR1H3 / LXR Alpha
Immunohistochemistry: LS-B3526 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3526 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Protective effects of farnesoid X receptor (FXR) on hepatic lipid accumulation are mediated by hepatic FXR and independent of intestinal FGF15 signal. Schmitt J, Kong B, Stieger B, Tschopp O, Schultze SM, Rau M, Weber A, M[Character fc]llhaupt B, Guo GL, Geier A. Liver international : official journal of the International Association for the Study of the Liver. Apr;35:1133-44. [Full Text Article] [PubMed:25156247] [PMC:PMC4146754]

About NR1H3 / LXR Alpha

Q13133 NM_005693 NP_005684.2

NR1H3 Antibody, Liver X receptor alpha Antibody, LXRA Antibody, LXR-a Antibody, Oxysterols receptor LXR-alpha Antibody, LXR alpha Antibody, Lxralpha Antibody, RLD-1 Antibody

Liver X receptor alpha (LXR alpha), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect the transport and catabolism of cholesterol as well as fatty acid metabolism. LXR alpha also appears to be an important insulin mediating factor in the regulation of lipogenesis.

Immunohistochemistry

Immunohistochemistry
Anti-NR1H3 / LXR Alpha antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3526 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NR1H3 / LXR Alpha antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3526 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NR1H3 / LXR Alpha antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3526 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/3/2016

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