Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species.
Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml]
NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2.
Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/ml as a capture antibody.
Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody (M01), clone 1E3-A6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Requested From: United States
Date Requested: 2/28/2017