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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NQO1

NAD(P)H dehydrogenase, quinone 1

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Name: NAD(P)H dehydrogenase, quinone 1
Synonyms: NQO1, Azoreductase, DHQU, DT-diaphorase, Dioxin-inducible 1, DIA4, Diaphorase-4, NAD(P)H:quinone oxireductase, Phylloquinone reductase, QR1, Menadione reductase, NMOR1, NMORI, DTD, Quinone reductase 1
Target Sequences: NM_000903 NP_000894.1 P15559

Publications (15)

1
Immortalisation of a human diploid fibroblast cell strain: a DT-diaphorase paradox. Kuehl BL, Brezden CB, Traver RD, Siegel D, Ross D, Renzing J, Rauth AM. The British journal of cancer. Supplement. 1996 27:S19-22. (WB; Human) [PubMed:8763839] [PMC:PMC2150025]
2
Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues. Siegel D, Ross D. Free radical biology & medicine. 2000 29:246-53. [PubMed:11035253]
3
Rapid polyubiquitination and proteasomal degradation of a mutant form of NAD(P)H:quinone oxidoreductase 1. Siegel D, Anwar A, Winski SL, Kepa JK, Zolman KL, Ross D. Molecular pharmacology. 2001 59:263-8. (WB, IP; Human) [PubMed:11160862]
4
Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems. Anwar A, Dehn D, Siegel D, Kepa JK, Tang LJ, Pietenpol JA, Ross D. The Journal of biological chemistry. 2003 278:10368-73. (WB, IP; Human) [PubMed:12529318]
5
Association of NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population. Zhang J, Schulz WA, Li Y, Wang R, Zotz R, Wen D, Siegel D, Ross D, Gabbert HE, Sarbia M. Carcinogenesis. 2003 24:905-9. (IHC-P; Human) [PubMed:12771035]
6
trans-Stilbene oxide induces expression of genes involved in metabolism and transport in mouse liver via CAR and Nrf2 transcription factors. Slitt AL, Cherrington NJ, Dieter MZ, Aleksunes LM, Scheffer GL, Huang W, Moore DD, Klaassen CD. Molecular pharmacology. 2006 69:1554-63. [PubMed:16449384]
7
5-Methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione, a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1, exhibits activity against human pancreatic cancer in vitro and in vivo. Dehn DL, Siegel D, Zafar KS, Reigan P, Swann E, Moody CJ, Ross D. Molecular cancer therapeutics. 2006 5:1702-9. (WB; Human) [PubMed:16891456]
8
Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury. Lee JC, Bhora F, Sun J, Cheng G, Arguiri E, Solomides CC, Chatterjee S, Christofidou-Solomidou M. American journal of physiology. Lung cellular and molecular physiology. 2008 294:L255-65. [PubMed:18083772]
9
Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus disease. Cho HY, Imani F, Miller-DeGraff L, Walters D, Melendi GA, Yamamoto M, Polack FP, Kleeberger SR. American journal of respiratory and critical care medicine. 2009 179:138-50. [PubMed:18931336] [PMC:PMC2633060]
10
Effect of tumor necrosis factor-alpha on estrogen metabolism and endometrial cells: potential physiological and pathological relevance. Salama SA, Kamel MW, Diaz-Arrastia CR, Xu X, Veenstra TD, Salih S, Botting SK, Kumar R. The Journal of clinical endocrinology and metabolism. 2009 94:285-93. [PubMed:18957495] [PMC:PMC2630861]
11
Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells. Li CQ, Kim MY, Godoy LC, Thiantanawat A, Trudel LJ, Wogan GN. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:14547-51. [PubMed:19706542] [PMC:PMC2732862]
12
Heightened endoplasmic reticulum stress in the lungs of patients with chronic obstructive pulmonary disease: the role of Nrf2-regulated proteasomal activity. Malhotra D, Thimmulappa R, Vij N, Navas-Acien A, Sussan T, Merali S, Zhang L, Kelsen SG, Myers A, Wise R, Tuder R, Biswal S. American journal of respiratory and critical care medicine. 2009 180:1196-207. [PubMed:19797762] [PMC:PMC2796732]
13
Nrf2 and Keap1 abnormalities in non-small cell lung carcinoma and association with clinicopathologic features. Solis LM, Behrens C, Dong W, Suraokar M, Ozburn NC, Moran CA, Corvalan AH, Biswal S, Swisher SG, Bekele BN, Minna JD, Stewart DJ, Wistuba II. Clinical cancer research : an official journal of the American Association for Cancer Research. 2010 16:3743-53. (IHC-P; Human) [PubMed:20534738] [PMC:PMC2920733]
14
Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system. Gao M, Singh A, Macri K, Reynolds C, Singhal V, Biswal S, Spannhake EW. Respiratory research. 2011 12:92. [PubMed:21752292] [PMC:PMC3154159]
15
Histone deacetylase inhibition activates transcription factor Nrf2 and protects against cerebral ischemic damage. Wang B, Zhu X, Kim Y, Li J, Huang S, Saleem S, Li RC, Xu Y, Dore S, Cao W. Free radical biology & medicine. 2012 52:928-36. (WB; Mouse) [PubMed:22226832]
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Target
NQO1 (90)
Reactivity
Human (69)
Mouse (25)
Rat (33)
Bovine (9)
Chimpanzee (6)
Dog (8)
Gibbon (6)
Horse (5)
Monkey (12)
Opossum (1)
Orangutan (6)
Pig (4)
Primate (5)
Rabbit (5)
Application
IHC (55)
IHC-P (18)
WB (86)
Flo (8)
ELISA (14)
ICC (7)
IF (13)
IP (19)
Peptide-ELISA (3)
RNAi (1)
Host
rabbit (65)
mouse (23)
goat (2)
Isotype
IgG (16)
IgG1 (21)
IgG1,k (1)
IgG2a,k (1)
Clonality
monoclonal mc (23)
polyclonal pc (67)
Clone
1A11 (2)
1E3-A6 (1)
4D12 (2)
A180 (8)
AI80 (2)
Skiny-1 (1)
Format
AP Conjugated (1)
APC (3)
APC Conjugated (3)
Biotin Conjugated (11)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (10)
HRP Conjugated (8)
PE Conjugated (4)
Unconjugated (44)
Epitope
aa2-274 (20)
aa2-273 (10)
C-Terminus (7)
aa111-160 (4)
C-terminal (1)
N-Terminus (1)
Phe252 (1)
aa118-144 (1)
aa134-146 (1)
aa203-252 (1)
aa213-274 (1)
aa242-274 (1)
aa250-274 (1)
aa263-274 (1)
Publications
No (90)
Showing 1-25 of 90 results

NQO1 Antibody (aa263-274) IHC-plus™ LS-B11145
Goat Polyclonal to Human NQO1
Rabbit, Mouse, Dog, Human, Monkey
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-NQO1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NQO1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

NQO1 Antibody (clone A180) IHC-plus™ LS-B116
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Dog, Rat, Primate, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $425.00
Anti-NQO1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

NQO1 Antibody (clone A180, Biotin) LS-C142021
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Mouse, Dog, Rat, Primate, Human
IF IHC IHC-P IP WB
Biotin Conjugated
100µl  $365.00
Anti-NQO1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NQO1 in A549 lysates. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NQO1 Antibody (clone A180, DY650) LS-C142022
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Dog, Rat, Primate, Human
IF IHC IHC-P IP WB
DY650 Conjugated
100µl  $365.00
Anti-NQO1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NQO1 in A549 lysates. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NQO1 Antibody (clone A180, DY550) LS-C142025
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Dog, Rat, Primate, Human
IF IHC IHC-P IP WB
DY550 Conjugated
100µl  $365.00
Anti-NQO1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NQO1 in A549 lysates. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NQO1 Antibody (clone A180, DY488) LS-C142023
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Dog, Rat, Primate, Human
IF IHC IHC-P IP WB
DY488 Conjugated
100µl  $365.00
Anti-NQO1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NQO1 in A549 lysates. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

NQO1 Antibody (aa134-146) IHC-plus™ LS-B8354
Goat Polyclonal to Human NQO1
Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
NQO1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

NQO1 Antibody LS-C47359
Rabbit Polyclonal to Human NQO1
Mouse, Dog, Bovine, Rat, Opossum, Horse, Human, Monkey
WB
Unconjugated
100µl  $335.00
Western blot of NQO1 in kidney lysate.  Lane 1 shows pre-immune sera and lane 2 show antibody tested on human kidney at 1:2500 dilution.

NQO1 Antibody (clone AI80) IHC-plus™ LS-B2886
Mouse Monoclonal [clone AI80] (IgG1) to Human NQO1
Rat, Human
IHC IHC-P IP WB
Unconjugated
50µl  $485.00
Anti-NQO1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-NQO1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

NQO1 Antibody LS-C405968
Rabbit Polyclonal (IgG) to Human NQO1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human liver cancer using NQO1 Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of paraffin-embedded Human ovarian cancer using NQO1 Polyclonal Antibody at dilution of 1:25.

NQO1 Antibody (clone A180) IHC-plus™ LS-B3275
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Human
IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-NQO1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NQO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

NQO1 Antibody (clone 4D12) LS-C169441
Mouse Monoclonal [clone 4D12] (IgG1) to Human NQO1
Human
ELISA Flo IHC IHC-P WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded testis tissues using NQO1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded ovarian cancer tissues using NQO1 mouse monoclonal antibody with DAB staining.

NQO1 Antibody LS-C754785
Rabbit Polyclonal (IgG) to Human NQO1
Human
ELISA Flo IF IHC IP WB
Unconjugated
100µg  $335.00

NQO1 Antibody (clone 1E3-A6) LS-C197049
Mouse Monoclonal [clone 1E3-A6] (IgG1,k) to Human NQO1
Human
ELISA IF IP RNAi WB
Unconjugated
100µg  $355.00
Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml]
NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2.

NQO1 Antibody (aa213-274) LS-C185348
Rabbit Polyclonal to Human NQO1
Human
ICC IHC IHC-P WB
Unconjugated
100µl 0.87mg/ml  $355.00
IHC of paraffin-embedded CL1-0 xenograft using NQO1 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using NQO1 antibody at 1:200 dilution.

NQO1 Antibody LS-C330772
Rabbit Polyclonal (IgG) to Human NQO1
Mouse, Human
Flo IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of K562 cells.
Western blot analysis of extracts of various cell lines, using NQO1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

NQO1 Antibody (clone 4D12) LS-C758984
Mouse Monoclonal [clone 4D12] (IgG1) to Human NQO1
Mouse, Human
Flo IHC WB
Unconjugated
100µl  $445.00
NQO1 Antibody in Immunohistochemistry (IHC)
NQO1 Antibody in Immunohistochemistry (IHC)

NQO1 Antibody LS-C73728
Mouse Monoclonal (IgG1) to Human NQO1
Rat, Human
IF IHC IHC-P IP WB
Unconjugated
100µl  $560.00

NQO1 Antibody LS-C678849
Rabbit Polyclonal (IgG) to Human NQO1
Human
ELISA IF IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human pancreatic cancer at dilution of 1:100
Immunofluorescent analysis of A549 cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NQO1 Antibody (aa118-144) LS-C100530
Rabbit Polyclonal to Human NQO1
Human
Flo IF WB
Unconjugated
400µl  $325.00
Confocal immunofluorescence of NQO1 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of NQO1 antibody in NCI-H460 cell line lysates (35 ug/lane). NQO1 (arrow) was detected using the purified antibody.

NQO1 Antibody (clone A180) LS-C188428
Mouse Monoclonal [clone A180] (IgG1) to Human NQO1
Rat, Human
IHC IHC-P IP WB
Unconjugated
0.1mg  $325.00

NQO1 Antibody LS-C122244
Mouse Monoclonal (IgG1) to Human NQO1
Rat, Human
IHC IHC-P IP WB
Unconjugated
100µg  $515.00

NQO1 Antibody (clone AI80) LS-C82339
Mouse Monoclonal [clone AI80] (IgG1) to Human NQO1
Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl  $450.00
Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded kidney tissue

NQO1 Antibody (clone 1A11) LS-C169442
Mouse Monoclonal [clone 1A11] (IgG1) to Human NQO1
Human
ELISA Flo WB
Unconjugated
100µl  $365.00
Western blot using NQO1 monoclonal antibody against human NQO1 (AA: 134-274) recombinant protein. (Expected MW is 41.3 kDa)
Western blot using NQO1 mouse monoclonal antibody against A549 (1), HeLa (2), MCF-7 (3) and HepG2 (4) cell lysate.

NQO1 Antibody LS-C402727
Rabbit Polyclonal (IgG) to Human NQO1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
120µl 1.6mg/ml  $335.00
Immunohistochemistry of paraffin-embedded mouse kidney using NQO1 Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human lung cancer using NQO1 Polyclonal Antibody at dilution of 1:40.
Showing 1-25 of 90 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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