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Anti-NPHS1 / Nephrin Antibody (aa1243-1256) LS-C37498

Catalog Size Price
LS-C37498-100 100 µl Unavailable

Most Popular NPHS1 / Nephrin Antibodies

Anti-NPHS1 / Nephrin Antibody (aa1218-1234) IHC-plus™ LS-B106
Rabbit Polyclonal to Human NPHS1 / Nephrin
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NPHS1 / Nephrin Antibody (aa1243-1256) LS-C37498
Guinea pig Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-NPHS1 / Nephrin Antibody (C-Terminus) IHC-plus™ LS-B1382
Rabbit Polyclonal to Human NPHS1 / Nephrin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NPHS1 / Nephrin Antibody (aa1243-1256) LS-C86180
Guinea pig Polyclonal to Human NPHS1 / Nephrin
Human
IHC, Immunofluorescence
Unconjugated
Anti-NPHS1 / Nephrin Antibody (aa37-1049) LS-C150221
Goat Polyclonal (IgG) to Mouse NPHS1 / Nephrin
Mouse
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Guinea pig Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human NPHS1 / Nephrin
Guinea pig
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • Western blot (1:250 - 1:500)
  • ELISA

Specificity and Use

NPHS1 / Nephrin antibody was raised against synthetic peptide (intracellular domain, aa1243-1256) of Nephrin.
aa1243-1256
Recognizes nephrin, first described as a kidney podocyte marker protein (MW 135kD calculated from amino acid sequence data; apparent Mr 185kD after SDS-PAGE).
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Immunohistochemistry (Paraffin): 1:25. Microwave antigen retrieval required. Immunofluorescence: Frozen tissue. Western Blot: 1:250; 1 hr at RT or overnight at 4?C.

Packaging

PBS, 0.09% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About NPHS1 / Nephrin

O60500 NM_004646 NP_004637.1

NPHS1 Antibody, Nephrin Antibody, NPHN Antibody, CNF Antibody

NPHS1 / Nephrin is a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.

Immunofluorescence

Immunofluorescence
Immunofluorescence of cultured mouse podocyte cells for nephrin

Requested From: 
Date Requested: 6/26/2017

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