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Anti-NOXO1 Antibody (aa238-252) IHC-plus™ LS-B485

Note: This antibody replaces LS-C19121

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NOXO1 Antibodies

Anti-NOXO1 Antibody (aa363-376) IHC-plus™ LS-B3
Goat Polyclonal to Human NOXO1
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOXO1 Antibody (aa238-252) IHC-plus™ LS-B485
Rabbit Polyclonal to Human NOXO1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOXO1 Antibody (aa192-219) LS-C158415
Rabbit Polyclonal to Human NOXO1
Human
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NOXO1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human NOXO1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 10 µg/ml)
  • Immunofluorescence
  • Western blot (1:2000 - 1:8000)
  • ELISA (1:20000 - 1:100000)

Specificity and Use

NOXO1 antibody was raised against recombinant human NOXO1.
aa238-252
Amino acids 238-252 of human NOXO1 protein.
Immunohistochemistry: LS-B485 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B485 was determined to be 5-10 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NOXO1

Q8NFA2 NM_144603 NP_751908.1

NOXO1 Antibody, NADPH oxidase organizer 1 Antibody, Nox-organizing protein 1 Antibody, p41NOX Antibody, p41NOXA Antibody, p41NOXC Antibody, Regulatory protein P41NOX Antibody, Nox organizer 1 Antibody, p41NOXB Antibody, SH3PXD5 Antibody, SNX28 Antibody

Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.

Immunohistochemistry

Immunohistochemistry
Anti-NOXO1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B485 concentration 10 ug/ml.

Western blot

Western blot
Anti-NOXO1 Antibody - Western Blot. Western blot of Affinity Purified anti-NOXO1 antibody shows detection of a band ~50 kD corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1:1000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Personal Communication, Zhenggang Liu, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 12/9/2016

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