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Anti-NOTCH1 Antibody (aa1754-1766) LS-C16929


Wt. Vol. Conc. Price
- 100 µl - $425
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NOTCH1 Antibodies

Anti-NOTCH1 Antibody (aa2488-2502) IHC-plus™ LS-B398
Rabbit Polyclonal to Human NOTCH1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-NOTCH1 Antibody (clone 3E12) LS-C114369
Mouse Monoclonal [clone 3E12] (IgG2b) to Human NOTCH1
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-NOTCH1 Antibody (Val1754) LS-C178239
Rabbit Polyclonal to Human NOTCH1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NOTCH1
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse
Western blot, ELISA


Human NOTCH1
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)


  • Western blot (1:250)
  • ELISA (1:10000 - 1:30000)

Specificity and Use

NOTCH1 antibody was raised against a synthetic peptide, VLLSRKRRRQHGQC, corresponding to amino acid residues 1755-1767 of human Notch 1. This is the N-terminal sequence of the cleaved N intracellular domain (NICD). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse (100%); Bat (92%); Opossum, Chicken, Trout, Stickleback (85%).
Recognizes human NOTCH 1. Species cross-reactivity: mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. Species sequence homology: mouse and rat NOTCH 1-100%. The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. No reactivity is detected against mouse N2, N3 or N4.
Suitable for use in ELISA and Western Blot. ELISA: 1:10000-1:30000. Standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb. and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:250. Against recombinant mouse Notch constructs in 293 cells. An 80kD band corresponding to Notch 1.


PBS, 0.1% sodium azide, 40% glycerol. No stabilizing proteins added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NOTCH1

P46531 NM_017617 NP_060087.3

NOTCH1 Antibody, Notch 1 Antibody, TAN1 Antibody

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Requested From: United States
Date Requested: 10/26/2016

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