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Anti-NOD2 / CARD15 Antibody (C-Terminus) IHC-plus™ LS-B2721

Note: This antibody replaces LS-C19465, LS-C56774, LS-C42334

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Related Products

Most Popular NOD2 / CARD15 Antibodies

Anti-NOD2 / CARD15 Antibody (aa28-301, clone 2D9) LS-C2580
Mouse Monoclonal [clone 2D9] (IgG1) to Human NOD2 / CARD15
Human, Mouse
IHC
Unconjugated
Western blot Image
Anti-NOD2 / CARD15 Antibody (N-Terminus) IHC-plus™ LS-B2720
Rabbit Polyclonal to Human NOD2 / CARD15
Human
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOD2 / CARD15 Antibody (C-Terminus) IHC-plus™ LS-B2721
Rabbit Polyclonal to Human NOD2 / CARD15
Human
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NOD2 / CARD15
Human
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

Details

Human NOD2 / CARD15
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (2 - 4 µg/ml)
  • ELISA

Specificity and Use

NOD2 / CARD15 antibody was raised against synthetic peptide from human NOD2.
LS-E29984 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B2721. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2
Immunohistochemistry: LS-B2721 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2721 was determined to be 3 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About NOD2 / CARD15

Q9HC29 NM_022162 NP_071445.1

NOD2 Antibody, BLAU Antibody, CARD15 Antibody, ACUG Antibody, NOD-like receptor C2 Antibody, NOD2B Antibody, NLRC2 Antibody, IBD1 Antibody, CLR16.3 Antibody, PSORAS1 Antibody

Recognizes muramyl dipeptide (MDP) constituents of bacterial peptidoglycans and plays a key role in gastrointestinal immunity: upon stimulation, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling, leading to activate the transcription of hundreds of genes involved in immune response.

Immunohistochemistry

Immunohistochemistry
Anti-NOD2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2721 concentration 3 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 µg/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 ug/ml.

Requested From: 
Date Requested: 3/24/2017

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