LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
NKX2‑5 Antibody (clone 1E4‑G5) LS‑C196993
NKX2-5 antibody LS-C196993 is an unconjugated mouse monoclonal antibody to human NKX2-5. Validated for ELISA and WB.
Catalog
Size
Price
LS-C196993-100
100 µg
Unavailable

Popular NKX2-5 Products

IHC of NKX2-5 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148622 (bottom left, right) at 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of paraformaldehyde-fixed HeLa using NKX2-5 antibody at 1:200 dilution.
Species: Human
Applications: ICC, Western blot
Anti-NKX2-5 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

NKX2-5 antibody LS-C196993 is an unconjugated mouse monoclonal antibody to human NKX2-5. Validated for ELISA and WB.
About NKX2-5
Nkx 2.5 is a lineage restricted transcription factor that regulates the expression of heart specific genes. This regulation is essential for tissue differentiation and the determination of temporal and spatial patterns of development. Abundant expression of Nkx 2.5 is observed in the mouse heart from the time of cardiac differentiation through adult life. P52952 NM_004387 NP_004378.1

NKX2-5 Antibody, Cardiac-specific homeo box Antibody, Cardiac-specific homeobox 1 Antibody, Cardiac-specific homeobox Antibody, CSX1 Antibody, CHNG5 Antibody, HLHS2 Antibody, NKX2E Antibody, NKX4-1 Antibody, NKX2.5 Antibody, Tinman paralog Antibody, VSD3 Antibody, CSX Antibody, Homeobox protein CSX Antibody, Homeobox protein Nkx-2.5 Antibody, NK2 homeobox 5 Antibody


Specifications

Target
Human NKX2-5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1E4-G5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
NKX2-5 antibody was raised against nKX2-5 (AAH25711, aa1-324 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human NKX2-5
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.
NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).
NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.
NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).
NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.
NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).
NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M01), clone 1E4-G5.Lane 1: NKX2-5 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.
NKX2-5 monoclonal antibody (M01), clone 1E4-G5 Western Blot analysis of NKX2-5 expression in HeLa.

Western blot

NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).
NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NKX2-5 is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number