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Catalog Number Size Price
LS-C112856-100 100 µg (0.5 mg/ml) $503 
NKX2-5 Antibody - CSX1 / NKX2-5 antibody (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NKX2-5 Antibody - CSX1 / NKX2-5 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NKX2-5 Antibody - NKX2-5 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NKX2-5 Antibody - CSX1 / NKX2-5 antibody (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NKX2-5 Antibody - CSX1 / NKX2-5 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NKX2-5 Antibody - NKX2-5 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Polyclonal Goat anti‑Human NKX2‑5 Antibody (aa94‑107, IHC, IF, WB) LS‑C112856

Polyclonal Goat anti‑Human NKX2‑5 Antibody (aa94‑107, IHC, IF, WB) LS‑C112856

Antibody:
NKX2-5 Goat anti-Human Polyclonal (aa94-107) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C112856-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NKX2-5 Goat anti-Human Polyclonal (aa94-107) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
NKX2-5 antibody LS-C112856 is an unconjugated goat polyclonal antibody to NKX2-5 (aa94-107) from human. It is reactive with human and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human NKX2-5
Synonyms
NKX2-5 | Cardiac-specific homeo box | Cardiac-specific homeobox 1 | Cardiac-specific homeobox | CSX1 | CHNG5 | HLHS2 | NKX2E | NKX4-1 | NKX2.5 | Tinman paralog | VSD3 | CSX | Homeobox protein CSX | Homeobox protein Nkx-2.5 | NK2 homeobox 5
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1NP_001159647.1NP_001159648.1.
Epitope
aa94-107
Specificity
Human NKX2-5. This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NKX2-5
P52952 NM_004387 NP_004378.1

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Requested From: United States
Date Requested: 4/24/2024