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Anti-NFYA Antibody (N-Terminus) LS-C18769


Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular NFYA Antibodies

Anti-NFYA Antibody (N-Terminus) IHC-plus™ LS-B131
Rabbit Polyclonal to Human NFYA
IHC - Paraffin, Western blot, ELISA, Gel shift
Immunohistochemistry Image
Anti-NFYA Antibody (C-Terminus) LS-C31566
Rabbit Polyclonal to Human NFYA
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-NFYA Antibody (aa333-347) LS-C288861
Rabbit Polyclonal (IgG) to Human NFYA
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NFYA
Western blot, ELISA


Human NFYA
Ion exchange chromatography


  • Western blot (1:1000)
  • ELISA (10,000)

Specificity and Use

NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kD forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. No reaction was observed by immunoblot against the 25 kD B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:10000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.


0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Long term: -70C; Short term: -20C.
For research use only.

About NFYA

P23511 NM_021705 NP_068351.1

NFYA Antibody, CBF-A Antibody, CBF-B Antibody, HAP2 Antibody, HAP2 CCAAT-binding protein Antibody, NF-YA Antibody

NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit.

Western blot

Western blot
Anti-NF-Y(A subunit) Antibody - Western Blot. CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and western blot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30 exposure. The antibody recognizes NF-YA as a doublet of ~42 kD in size.

Requested From: 
Date Requested: 3/29/2017

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