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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFE2L2 / NRF2 Antibody (aa445‑458) LS‑C154863
NRF2 antibody LS-C154863 is an unconjugated goat polyclonal antibody to NRF2 (NFE2L2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C154863-100
100 µg (0.5 mg/ml)
Unavailable

Popular NFE2L2 / NRF2 Products

NFE2L2 / NRF2 antibody LS-C31314 Western blot of A172 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Pig
Applications: IHC, ICC, Western blot, Immunoprecipitation, ELISA, Competition
Western blot of Nrf2(Nfe2l2)(arrow) using rabbit polyclonal Nrf2(Nfe2l2) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2) (Origene Technologies).
Species: Human
Applications: Western blot
Western blot of NFE2L2-S40 in T47D(lane 1) and 293(lane 2) cell line lysates (35 ug/lane). NFE2L2 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot
IHC of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

NRF2 antibody LS-C154863 is an unconjugated goat polyclonal antibody to NRF2 (NFE2L2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About NFE2L2 / NRF2
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Q16236 NM_006164 NP_006155.2

NFE2L2 Antibody, NF-E2-related factor 2 Antibody, NFE2-related factor 2 Antibody, NRF2 Antibody


Specifications

Target
Human NFE2L2 / NRF2
Host
Goat
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster (tested or 100% immunogen sequence identity)
Predicted
Monkey, Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
NFE2L2 / NRF2 antibody was raised against synthetic peptide C-KHSSRLEAHLTRDE from an internal region of human NRF2 (NP_006155.2; NP_001138884.1; NP_001138885.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Dog, Bat, Bovine, Dolphin, Hamster, Panda, Porcine (100%); Marmoset, Elephant, Horse, Rabbit, Guinea pig (93%); Turkey, Chicken (86%).
Blocking Peptide
LS-E27736 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154863. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa445-458
Specificity
Human NRF2. This antibody is expected to recognize all reported isoforms (NP_006155.2; NP_001138884.1; NP_001138885.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70kD band observed in Human Lung lysates (calculated MW of 67.8kD according to NP_006155.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NFE2L2 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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