Pig, Human, Mouse, Rat, Bovine, Avian, Mammal
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- IHC - Frozen
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NF-L / NEFL antibody was raised against native NF-L purified from pig spinal cord
Specifically recognizes the light neurofilament subunit (~68 kD).
Immunohistochemistry: LS-B257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B257 was determined to be 2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.