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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NF‑L / NEFL Antibody (clone DA2) LS‑C2725
NEFL antibody LS-C2725 is an unconjugated mouse monoclonal antibody to NEFL (NF-L) from human, mouse, rat and other species. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C2725-50
50 µl
$335
Description
NEFL antibody LS-C2725 is an unconjugated mouse monoclonal antibody to NEFL (NF-L) from human, mouse, rat and other species. Validated for IF, IHC and WB.
Target
Pig NF-L / NEFL
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Avian, Mammal (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [DA2]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Fixed
  • Immunofluorescence
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NF-L / NEFL antibody was raised against nF-L purified from pig spinal cord
Specificity
Specifically recognizes the light neurofilament subunit or NF-L (~68 kD).
Presentation
0.1% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About NF-L / NEFL
P07196 NM_006158 NP_006149.2

Popular NF-L / NEFL Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Pig, Human, Mouse, Rat, Bovine, Avian, Mammal
Applications: IHC, IHC - Paraffin, IHC - Frozen
.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

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Images

Immunofluorescence

Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).

Immunofluorescence

Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).

Immunofluorescence

Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).

Immunofluorescence

Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.
Neurofilament staining of cultured neurons (green) with the Neurofilament light chain antibody. Nuclei are stained blue.

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFL / NF-L Antibody Neurofilament light chain Antibody (NF-L ) (lane 4).

Requested From: United States
Date Requested: 12/10/2018
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