Human, Monkey, Mouse, Rat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Bat, Bovine, Guinea pig, Horse, Pig (at least 90% immunogen sequence identity)
Western blot (0.2 - 1 µg/ml)
Specificity and Use
NEXN / Nexilin antibody was raised against synthetic peptide located between aa252-301 of human NEXN (Q0ZGT2, NP_653174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit, Platypus (100%); Galago, Bovine, Bat, Horse, Pig, Guinea pig (92%); Elephant, Panda, Dog (85%); Xenopus (84%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Lyophilized from PBS with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.