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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NELFE / RD / RDBP Antibody (aa35‑84) LS‑C110517
RDBP antibody LS-C110517 is an unconjugated rabbit polyclonal antibody to RDBP (NELFE / RD) from human, mouse and monkey. Validated for WB.
Catalog
Size
Price
LS-C110517-50
50 µg
Unavailable

Popular NELFE / RD / RDBP Products

Detection of Human NELFE by Immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-NELFE used at a dilution of 1:250.
Species: Human, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human NELFE by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NELFE used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human lung cancer tissue.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemical analysis of NELF-E staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

RDBP antibody LS-C110517 is an unconjugated rabbit polyclonal antibody to RDBP (NELFE / RD) from human, mouse and monkey. Validated for WB.
About NELFE / RD / RDBP
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. P18615 NM_002904 NP_002895.3

NELFE Antibody, D6S45 Antibody, Nuclear protein Antibody, RD RNA binding protein Antibody, RD Antibody, RD RNA-binding protein Antibody, Negative elongation factor E Antibody, NELF-E Antibody, RDBP Antibody, RNA-binding protein RD Antibody, RDP Antibody


Specifications

Target
Human NELFE / RD / RDBP
Host
Rabbit
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa35-84 of human NELFE (P18615, NP_002895). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse (100%); Galago, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (92%).
Blocking Peptide
LS-E14745 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110517. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human RDBP
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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