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Anti-NEK2 Antibody (aa287-299) IHC-plus™ LS-B129

Note: This antibody replaces LS-C18891


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NEK2 Antibodies

Anti-NEK2 Antibody (aa287-299) IHC-plus™ LS-B129
Rabbit Polyclonal to Human NEK2
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-NEK2 Antibody (clone 2F6) IHC-plus™ LS-B5478
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human NEK2
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Image
Anti-NEK2 Antibody (aa396-426) IHC-plus™ LS-B11803
Rabbit Polyclonal to Human NEK2
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEK2
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human NEK2
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

NEK2 antibody was raised against synthetic peptide from human NEK2.
aa 287-299 of Human NEK2 protein.
Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About NEK2

P51955 NM_002497 NP_002488.1

NEK2 Antibody, NIMA-related kinase 2 Antibody, NEK2A Antibody, HsPK21 Antibody, NimA-like protein kinase 1 Antibody, NLK1 Antibody, HSPK 21 Antibody, NimA-related protein kinase 2 Antibody

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes.


Anti-NEK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B129 concentration 2.5 ug/ml.


Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B129 concentration 2.5 ug/ml.

Western blot

Western blot
Anti-NEK2 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kD corresponding to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

Requested From: United States
Date Requested: 10/23/2016

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