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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEDD8 Antibody LS‑C9225
NEDD8 antibody LS-C9225 is an unconjugated rabbit polyclonal antibody to yeast NEDD8. Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C9225-500
500 µg
$830

Popular NEDD8 Products

Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6641 dilution 1:100.
Species: Human, Mouse, Rat
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IHC of paraffin-embedded cervical cancer tissues using NEDD8 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Anti-NEDD8 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11079 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot analysis of extracts of HeLa cells, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry

Product Description

NEDD8 antibody LS-C9225 is an unconjugated rabbit polyclonal antibody to yeast NEDD8. Validated for ELISA and WB.
About NEDD8
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Q15843 NM_006156 NP_006147.1

NEDD8 Antibody, NEDD-8 Antibody, Neddylin Antibody, Ubiquitin-like protein Nedd8 Antibody


Specifications

Target
Yeast NEDD8
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • ELISA (1:1000 - 1:5000)
Immunogen
NEDD8 antibody was raised against full-length recombinant yeast Rub1 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:5000. Western Blot: 1:500 (6kD).
Presentation
Lyophilized
Reconstitution
500 µl deionized water
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/24/2018

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