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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEDD8

neural precursor cell expressed, developmentally down-regulated 8

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Gene Name: neural precursor cell expressed, developmentally down-regulated 8
Synonyms: NEDD8, NEDD-8, Neddylin, Ubiquitin-like protein Nedd8
Target Sequences: NM_006156 NP_006147.1 Q15843

Filtered By: None
Target
NEDD8 (38)
Reactivity
Human (37)
Mouse (15)
Rat (12)
Bovine (2)
Dog (1)
Monkey (3)
Rabbit (1)
Xenopus (1)
Yeast (1)
Application
IHC (25)
IHC-Fr (1)
IHC-P (10)
WB (38)
Flo (4)
ELISA (22)
ICC (1)
IF (6)
IP (3)
Peptide-ELISA (1)
Host
rabbit (32)
mouse (5)
sheep (1)
Isotype
IgG (27)
IgG1 (2)
IgG2b (2)
Clonality
monoclonal mc (5)
polyclonal pc (33)
Clone
1A7 (1)
5B8 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (2)
Unconjugated (26)
Epitope
aa1-32 (8)
N-Terminus (4)
aa1-81 (2)
10-90 aa, N-terminal (1)
Asp21 (1)
aa10-59 (1)
aa2-77 (1)
aa20-32 (1)
Publications
No (37)
Yes (1)
Showing 1-25 of 38 results

NEDD8 Antibody (aa10-59) IHC-plus™ LS-B6641
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.
Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.

NEDD8 Antibody (clone 5B8) IHC-plus™ LS-B11079
Mouse Monoclonal [clone 5B8] (IgG1) to Human NEDD8
Human
ELISA Flo IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-NEDD8 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Anti-NEDD8 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

NEDD8 Antibody (aa20-32) IHC-plus™ LS-B4950
Mouse Monoclonal (IgG2b) to Human NEDD8
Human
ELISA IHC IHC-P WB
Unconjugated
50µl 2.6mg/ml  $495.00
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

NEDD8 Antibody (clone 1A7) LS-C169462
Mouse Monoclonal [clone 1A7] (IgG1) to Human NEDD8
Human
ELISA Flo IF IHC WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded cervical cancer tissues using NEDD8 mouse monoclonal antibody with DAB staining.
Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
IHC of paraffin-embedded cervical cancer tissues using NEDD8 mouse monoclonal antibody with DAB staining.
Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

NEDD8 Antibody LS-C408277
Mouse Monoclonal (IgG) to Human NEDD8
Human
Flo ICC IF IHC WB
Unconjugated
50µl  $285.00; 100µl  $375.00; 200µl  $495.00
Western blot analysis of extracts of HeLa cells, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of HeLa cells, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

NEDD8 Antibody (N-Terminus) LS-C37486
Rabbit Polyclonal to Human NEDD8
Xenopus, Rat, Mouse, Bovine, Human, Monkey
Flo IF IHC IHC-P IP WB
Unconjugated
100µl  $530.00
IHC of paraffin-embedded human colon carcinoma
Immunofluorescence of paraformaldehyde-fixed HeLa cells
IHC of paraffin-embedded human colon carcinoma
Immunofluorescence of paraformaldehyde-fixed HeLa cells

NEDD8 Antibody LS-C331310
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibodyat dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells.
Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibodyat dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells.

NEDD8 Antibody (aa1-81) LS-C377417
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Western blot. All lanes: NEDD8 antibody at 16 ug/ml. Lane 1: mouse heart tissue. Lane 2: mouse spleen tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 9 kDa. Observed band size: 9 kDa Immunohistochemistry.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Western blot. All lanes: NEDD8 antibody at 16 ug/ml. Lane 1: mouse heart tissue. Lane 2: mouse spleen tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 9 kDa. Observed band size: 9 kDa Immunohistochemistry.

NEDD8 Antibody (N-Terminus) LS-C94349
Sheep Polyclonal (IgG) to Mouse NEDD8
Mouse, Rat, Human
IHC IHC-Fr IHC-P WB
Unconjugated
500µg 500µl 1mg/ml  $325.00

NEDD8 Antibody (N-Terminus) LS-C352565
Rabbit Polyclonal to Human NEDD8
Rabbit, Mouse, Dog, Bovine, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of NEDD8 expression in BJAB (A); K562 (B); HeLa (C) whole cell lysates.
Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of NEDD8 expression in BJAB (A); K562 (B); HeLa (C) whole cell lysates.

NEDD8 Antibody LS-C660335
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
Unconjugated
100µg  $405.00

NEDD8 Antibody (aa1-32) LS-C328012
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

NEDD8 Antibody (aa1-32, Biotin) LS-C280020
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

NEDD8 Antibody (aa1-32, FITC) LS-C280022
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

NEDD8 Antibody (aa1-32, APC) LS-C280018
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

NEDD8 Antibody (aa1-32, AP) LS-C280019
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

NEDD8 Antibody (aa1-32, Azide-free, HRP) LS-C280021
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

NEDD8 Antibody (aa1-32, PE) LS-C280023
Rabbit Polyclonal (IgG) to Human NEDD8
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

NEDD8 Antibody (10-90 aa, N-terminal) LS-C384806
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Rat, Human, Monkey
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Neddylin antibody
Western blot of Neddylin antibody

NEDD8 Antibody LS-C661991
Rabbit Polyclonal to Human NEDD8
Human
IHC IHC-P WB
Unconjugated
100µg  $355.00
NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody
Western blot - Anti-NEDD8 Picoband Antibody
NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody
Western blot - Anti-NEDD8 Picoband Antibody

NEDD8 Antibody (aa1-32) LS-C98774
Rabbit Polyclonal to Human NEDD8
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of NEDD8 polyclonal antibody in HeLa cell lysate. NEDD8 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of NEDD8 polyclonal antibody in HeLa cell lysate. NEDD8 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

NEDD8 Antibody (Asp21) LS-C176339
Rabbit Polyclonal to Human NEDD8
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of NEDD8 (D21) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of NEDD8 (D21) pAb in extracts from Jurkat cells.
IHC of NEDD8 (D21) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of NEDD8 (D21) pAb in extracts from Jurkat cells.

NEDD8 Antibody LS-C748569
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Human, Monkey
IF WB
Unconjugated
50µl  $235.00

NEDD8 Antibody LS-C331019
Rabbit Polyclonal (IgG) to Human NEDD8
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $305.00; 200µl  $495.00
Western blot analysis of extracts of mouse brain, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of mouse brain, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

NEDD8 Antibody LS-C415967
Rabbit Polyclonal to Human NEDD8
Mouse, Rat, Human
IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of Jurkat cell lysate using NEDD8 Antibody
Western blot of Jurkat cell lysate using NEDD8 Antibody
Showing 1-25 of 38 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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