Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
NDUFA5 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 116 of NDUFA5 (SwissProt Q16718). Percent identity by BLAST analysis: Human (100%); Monkey (94%); Bovine (87%); Rat (80%).
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
NDUFA5 Antibody, B13 Antibody, Complex I 13kDa subunit B Antibody, CI-13KD-B Antibody, CI-13kB Antibody, Complex I subunit B13 Antibody, Complex I-13kD-B Antibody, NUFM Antibody, Type I dehydrogenase Antibody, Ubiquinone reductase Antibody, UQOR13 Antibody
The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain. The predicted protein is a 116 amino acid peptide with a molecular weight of 13.34 kDa. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The deduced peptide belongs to the 7 component-containing, water soluble ""iron-sulfur protein"" (IP) fraction of complex I, although its specific role is unknown.