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Anti-NCK1 / NCK Antibody (aa1-254) LS-C37474

Ordering

Wt. Vol. Conc. Price
100 µg - - $560
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NCK1 / NCK Antibodies

Anti-NCK1 / NCK Antibody (clone 11D10) IHC-plus™ LS-B7138
Mouse Monoclonal [clone 11D10] (IgG1,k) to Human NCK1 / NCK
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody IHC-plus™ LS-B7146
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody (aa62-92, Azide-free, HRP) LS-C223414
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated

Details

Human NCK1 / NCK
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation

Specificity and Use

NCK1 / NCK antibody was raised against purified GST fusion protein corresponding to amino acids 1 to 254 of human Nck, produced in E. coli.
aa1-254
Recognizes a human 47kD SH2/SH3-containing protein, Nck. Species cross-reactivity: Mouse and rat.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 ug immunoprecipitates Nck from a lysate of human A431 carcinoma cells, mouse 3T3/A31 fibroblasts and rat L6 skeletal fibroblasts.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
For research use only.

About NCK1 / NCK

P16333 NM_006153 NP_006144.1

NCK1 Antibody, Cytoplasmic protein nck Antibody, Cytoplasmic protein NCK1 Antibody, NCK adaptor protein 1 Antibody, Nck-1 Antibody, NCKalpha Antibody, Melanoma NCK protein Antibody, NCK Antibody, NCK tyrosine kinase Antibody

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Requested From: United States
Date Requested: 12/2/2016

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