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Anti-NANOG Antibody (Biotin) LS-C104594

Catalog Size Price
LS-C104594-25 25 µg Unavailable
LS-C104594-50 50 µg Unavailable

Related Products

121 NANOG Antibodies

Most Popular NANOG Antibodies

Anti-NANOG Antibody (aa24-73) IHC-plus™ LS-B1193
Rabbit Polyclonal to Human NANOG
Human, Monkey
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-NANOG antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1193 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NANOG Antibody (aa291-304) IHC-plus™ LS-B3017
Goat Polyclonal to Human NANOG
Human
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3017 concentration 2 ug/ml.
Anti-NANOG Antibody (aa94-123) IHC-plus™ LS-B3425
Rabbit Polyclonal to Human NANOG
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3425 concentration 10 ug/ml.
Anti-NANOG Antibody (clone 1E6C4) LS-C108845
Mouse Monoclonal [clone 1E6C4] (IgG1) to Human NANOG
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Confocal immunofluorescence of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Anti-NANOG Antibody (clone hNanog.2) IHC-plus™ LS-B7871
Mouse Monoclonal [clone hNanog.2] (IgG1,k) to Human NANOG
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7871 dilution 20 ug/ml.

100% Guaranteed
Rabbit Polyclonal to Human NANOG
Human
Western blot, ELISA
Biotin Conjugated

Details

Human NANOG
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

NANOG antibody was raised against e.coli derived recombinant Human Nanog.
Human Nanog
ELISA: To detect hNanog by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hNanog. To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Nanog (LS-B8225) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNanog. Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About NANOG

Q9H9S0 AB062943 NP_079141.2

NANOG Antibody, Nanog homeobox Antibody, NANOGP8 Antibody, HNanog Antibody, Homeobox protein NANOG Antibody

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Requested From: 
Date Requested: 5/24/2018

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