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Catalog Number Size Price
LS-B8225-50 50 µl (1 mg/ml) $485 
NANOG Antibody - Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
NANOG Antibody - Western Blot (reducing) of NANOG antibody
NANOG Antibody - Western Blot (non-reducing) of NANOG antibody
NANOG Antibody - Sandwich ELISA of NANOG antibody
NANOG Antibody - Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
NANOG Antibody - Western Blot (reducing) of NANOG antibody
NANOG Antibody - Western Blot (non-reducing) of NANOG antibody
NANOG Antibody - Sandwich ELISA of NANOG antibody
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IHC‑plus™ Polyclonal Rabbit anti‑Human NANOG Antibody (IHC, WB) LS‑B8225

IHC‑plus™ Polyclonal Rabbit anti‑Human NANOG Antibody (IHC, WB) LS‑B8225

Note: This antibody replaces LS-C104692, LS-C187914
Antibody:
NANOG Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B8225-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NANOG Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NANOG antibody LS-B8225 is an unconjugated rabbit polyclonal antibody to human NANOG. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NANOG
Synonyms
NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human Nanog.
Specificity
Human Nanog
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B8225 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Nanog (LS-C104594) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNanog. Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
50 µl Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NANOG
Q9H9S0 AB062943 NP_079141.2

Validation

NANOG Antibody - Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

LSBio Ratings

IHC-plus™ NANOG Antibody for IHC, WB/Western, ELISA LS-B8225 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/11/2024