(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 - 2 µg/ml)
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphory.
Suitable for use in Western Blot. Western Blot: 1-2 ug/ml.
PBS, 0.05% Sodium Azide, 0.05% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.