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Anti-MUTYH / MYH Antibody (aa531-546) IHC-plus™ LS-B1974

Note: This antibody replaces LS-C761, LS-C72933

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MUTYH / MYH Antibodies

Anti-MUTYH / MYH Antibody (aa531-546) IHC-plus™ LS-B1974
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MUTYH / MYH Antibody LS-C331584
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot Image
Anti-MUTYH / MYH Antibody LS-C332024
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human, Mouse
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human MUTYH / MYH
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

MUTYH / MYH antibody was raised against synthetic peptide from human MUTYH.
aa531-546
A synthetic peptide corresponding to amino acids 531-546 of human MYH.
Immunohistochemistry: LS-B1974 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1974 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MUTYH / MYH

Q9UIF7 NM_012222 NP_036354.1

MUTYH Antibody, HMYH Antibody, MYH Antibody, MutY (E. coli) homolog Antibody, CYP2C Antibody, MutY homolog Antibody, MutY homolog (E. coli) Antibody

MUTYH is a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-MUTYH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1974 concentration 10 ug/ml.

Western blot

Western blot
Western blot of MYH in 20 ugs of HeLa cell lysate using antibody at 2 ug/ml.

Requested From: United States
Date Requested: 12/9/2016

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