(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin
- Western blot (5 - 10 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
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MUC5B antibody was raised against synthetic peptide derived from the N-terminal region of the human MUC5B protein.
Specific for the calculated ~590kD MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 5-10 ug/ml. Immunohistochemistry (Formalin paraffin): 5-10 ug/ml. Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.
PBS, pH 7.4, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.