Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MUC5B antibody was raised against synthetic peptide derived from the N-terminal region of the human MUC5B protein.
Specific for the calculated ~590kD MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 5-10 ug/ml. Immunohistochemistry (Formalin paraffin): 5-10 ug/ml. Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.
PBS, pH 7.4, 0.1% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
MUC5B is a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus.