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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MUC5B Antibody (N‑Terminus, clone 5B19‑2E) LS‑C9123 Best Seller
MUC5B antibody LS-C9123 is an unconjugated mouse monoclonal antibody to human MUC5B. Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C9123-100
100 µg (0.5 mg/ml)
Unavailable

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Western blot of recombinant MUC5B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

MUC5B antibody LS-C9123 is an unconjugated mouse monoclonal antibody to human MUC5B. Validated for ELISA, IHC and WB.
About MUC5B
MUC5B is a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. Q9HC84 NM_002458 NP_002449.2

MUC5B Antibody, MUC-5B Antibody, MUC9 Antibody, Mucin-5B Antibody, Sublingual gland mucin Antibody, MG1 Antibody, Cervical mucin Antibody, Cervical mucin MUC5B Antibody, MUC5 Antibody


Specifications

Target
Human MUC5B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [5B19-2E]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (5 - 10 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MUC5B antibody was raised against synthetic peptide derived from the N-terminal region of the human MUC5B protein.
Epitope
N-Terminus
Specificity
Specific for the calculated ~590kD MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 5-10 ug/ml. Immunohistochemistry (Formalin paraffin): 5-10 ug/ml. Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.
Presentation
PBS, pH 7.4, 0.1% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/16/2018

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