Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 10B4] (IgG2b) to Human MTOR
IHC, IHC - Paraffin, Western blot
Rabbit Polyclonal to Human MTOR
Human, Mouse, Rat
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Western blot (0.1 - 1 µg/ml)
Specificity and Use
MTOR antibody was raised against synthetic peptide representing a portion of the protein encoded in part by exons 5 and 6 (LocusLink ID 1981).
Reacts with human, mouse and rat.
Western Blot: Overnight incubation at 4°C is recommended for primary antibody. MTOR requires detergent and extended Western Blot transfer times (e. g. in 5% acrylamide gels to PVDF, use 50V for 3 hours in CAPS buffer with 10% methanol and 0.1 % SDS).
Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide
Store at 2-8°C for up to 1 year. Avoid repeated freeze-thaw cycles.
MTOR Antibody, FRAP1 Antibody, FRAP2 Antibody, FRAP Antibody, Mammalian target of rapamycin Antibody, RAFT1 Antibody, Rapamycin and FKBP12 target 1 Antibody, RAPT1 Antibody, Rapamycin target protein 1 Antibody
Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2).