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Anti-MHC Class II Antibody (clone YKIX334.2, FITC) LS-C57164

Ordering

Wt. Vol. Conc. Price
- 100 tst - $445
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Rat Monoclonal [clone YKIX334.2] (IgG2a) to Dog MHC Class II
Dog
Flow Cytometry
FITC Conjugated

Details

Dog MHC Class II
Rat
Dog (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [YKIX334.2]
Fluorescein
Affinity purified
Unmodified

Applications

Flow Cytometry (1:1)

Specificity and Use

MHC Class II antibody was raised against con A activated canine peripheral blood cells.
Recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Is an antigen of ~32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.09% sodium azide, 1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MHC Class II

MHC (major histocompatibility complex) class II molecules are a family of molecules normally found only on antigen-presenting cells such as dendritic cells, mononuclear phagocytes, some endothelial cells, thymic epithelial cells, and B cells. The antigens presented by class II peptides are derived from extracellular proteins (not cytosolic as in class I); hence, the MHC class II-dependent pathway of antigen presentation is called the endocytic or exogenous pathway.

Flow Cytometry

Flow Cytometry
Staining of canine peripheral blood lymphocytes with rat anti-canine MHC CLASS II: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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