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Anti-MDM2 Antibody (aa154-167, clone SMP14) IHC-plus™ LS-B163

Note: This antibody replaces LS-C11561, LS-C43567, LS-C72325, LS-C25496, LS-C25498


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular MDM2 Antibodies

Anti-MDM2 Antibody (aa154-167, clone SMP14) IHC-plus™ LS-B163
Mouse Monoclonal [clone SMP14] (IgG1) to Human MDM2
Human, Monkey, Rat, Dog
IHC - Paraffin, IHC - Frozen, IHC - Resin, Immunoprecipitation
Immunohistochemistry Image
Anti-MDM2 Antibody (clone 1A7) IHC-plus™ LS-B5461
Mouse Monoclonal [clone 1A7] (IgG1,k) to Human MDM2
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-MDM2 Antibody (Internal) LS-C209837
Rabbit Polyclonal (IgG) to Zebrafish MDM2
IHC, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone SMP14] (IgG1) to Human MDM2
Human, Monkey, Rat, Dog
IHC - Paraffin, IHC - Frozen, IHC - Resin, Immunoprecipitation


Human MDM2
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Bat, Bovine, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
IgG1 Monoclonal [SMP14]
Purified IgG


  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • IHC - Resin
  • Immunoprecipitation

Specificity and Use

MDM2 antibody was raised against synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%); Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%); Mouse (86%).
Recognizes the 90kD MDM2 protein, which forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
Immunohistochemistry: LS-B163 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B163 was determined to be 10 ug/ml. Histology Positive Control Tissue: Breast Carcinoma.


PBS, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.
For research use only.

About MDM2

Q00987 NM_002392 NP_002383.2

MDM2 Antibody, ACTFS Antibody, Double minute 2 protein Antibody, Hdm2 Antibody, MDM2 variant FB30 Antibody, HDMX Antibody, Oncoprotein Mdm2 Antibody, p53-binding protein Mdm2 Antibody, MDM2 variant FB28 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein.


Anti-MDM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B163 concentration 10 ug/ml.

Requested From: United States
Date Requested: 10/27/2016

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