• Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-MCAM / CD146 Antibody (clone P1H12) IHC-plus™ LS-B4050

Note: This antibody replaces LS-C106635, LS-C14262
Catalog Size Price
LS-B4050-50 50 µg (0.5 mg/ml) Unavailable

Related Products

140 MCAM / CD146 Antibodies

Most Popular MCAM / CD146 Antibodies

Anti-MCAM / CD146 Antibody (aa19-560) LS-C35841
Mouse Monoclonal (IgG2a) to Rat MCAM / CD146
Western blot, Flow Cytometry, ELISA
Anti-MCAM / CD146 Antibody (Tyr641) IHC-plus™ LS-B3628
Rabbit Polyclonal to Human MCAM / CD146
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-MCAM / CD146 Antibody (clone P1H12) IHC-plus™ LS-B4050
Mouse Monoclonal [clone P1H12] (IgG1) to Human MCAM / CD146
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image
Anti-MCAM / CD146 Antibody LS-C149995
Goat Polyclonal (IgG) to Human MCAM / CD146
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-MCAM / CD146 Antibody (aa532-558, PE) LS-C262812
Rabbit Polyclonal (IgG) to Human MCAM / CD146
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed Publications (1)
Mouse Monoclonal [clone P1H12] (IgG1) to Human MCAM / CD146
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry


Human MCAM / CD146
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [P1H12]
Affinity purified


  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen (1 - 10 µg/ml)
  • Western blot (1 - 5 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

MCAM / CD146 antibody was raised against human umbilical vein cells
This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC cell line. This can be used at 0.25 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.


Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

Publications (1)

Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. Yazawa EM, Geddes-Sweeney JE, Cedeno-Laurent F, Walley KC, Barthel SR, Opperman MJ, Liang J, Lin JY, Schatton T, Laga AC, Mihm MC, Qureshi AA, Widlund HR, Murphy GF, Dimitroff CJ. The Journal of investigative dermatology. 2015 135:1849-1862. (WB; Human) [Full Text Article] [PubMed:25756799] [PMC:PMC4466041]

About MCAM / CD146

P43121 NM_006500 NP_006491.2

MCAM Antibody, CD146 Antibody, CD146 antigen Antibody, Gicerin Antibody, Melanoma adhesion molecule Antibody, MUC18 Antibody, p1h12 Antibody

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.


Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4050 concentration 20 ug/ml.

Flow Cytometry

Flow Cytometry
Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.

Requested From: 
Date Requested: 3/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy

Catalog Number