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Anti-MCAM / CD146 Antibody (clone P1H12) IHC-plus™ LS-B4050

Note: This antibody replaces LS-C106635, LS-C14262
Catalog Size Price
LS-B4050-50 50 µg (0.5 mg/ml) Unavailable

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157 MCAM / CD146 Antibodies

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Anti-MCAM / CD146 Antibody (aa433-647) LS-C33782
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Angioscarcoma stained with anti-CD146
Anti-MCAM / CD146 Antibody (aa19-560) LS-C35841
Mouse Monoclonal (IgG2a) to Rat MCAM / CD146
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Anti-MCAM / CD146 Antibody (Tyr641) IHC-plus™ LS-B3628
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Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3628 concentration 10 ug/ml.
Anti-MCAM / CD146 Antibody (clone P1H12) IHC-plus™ LS-B4050
Mouse Monoclonal [clone P1H12] (IgG1) to Human MCAM / CD146
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IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
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Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4050 concentration 20 ug/ml.
Anti-MCAM / CD146 Antibody (aa532-558, PE) LS-C262812
Rabbit Polyclonal (IgG) to Human MCAM / CD146
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IHC, Western blot, ELISA
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100% Guaranteed Publications (1)
Mouse Monoclonal [clone P1H12] (IgG1) to Human MCAM / CD146
Human
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human MCAM / CD146
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [P1H12]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen (1 - 10 µg/ml)
  • Western blot (1 - 5 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

MCAM / CD146 antibody was raised against human umbilical vein cells
This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC cell line. This can be used at 0.25 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Store at 4°C, avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. Yazawa EM, Geddes-Sweeney JE, Cedeno-Laurent F, Walley KC, Barthel SR, Opperman MJ, Liang J, Lin JY, Schatton T, Laga AC, Mihm MC, Qureshi AA, Widlund HR, Murphy GF, Dimitroff CJ. The Journal of investigative dermatology. 2015 135:1849-1862. (WB; Human) [Full Text Article] [PubMed:25756799] [PMC:PMC4466041]

About MCAM / CD146

P43121 NM_006500 NP_006491.2

MCAM Antibody, CD146 Antibody, CD146 antigen Antibody, Gicerin Antibody, Melanoma adhesion molecule Antibody, MUC18 Antibody, p1h12 Antibody

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.

Immunohistochemistry

Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4050 concentration 20 ug/ml.
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4050 concentration 20 ug/ml.

Flow Cytometry

Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.
Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.

Requested From: 
Date Requested: 5/20/2018

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