Human MBP / Myelin Basic Protein
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
- IHC - Paraffin (1:100)
- Western blot (1:1500)
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Human myelin basic protein from brain.
Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
Immunohistochemistry: LS-B3112 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3112 was determined to be 1:100. Western Blot: 1:1500 in TTBS, overnight at 4uC.
0.05M Tris HCL, pH 7.6, 15 mM sodium azide before the addition of glycerol to 40%
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.