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Anti-MAPKAPK5 / PRAK Antibody (aa306-318) IHC-plus™ LS-B9567

Note: This antibody replaces LS-C186552

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPKAPK5 / PRAK Antibodies

Anti-MAPKAPK5 / PRAK Antibody LS-C41490
Mouse Monoclonal (IgG1) to Human MAPKAPK5 / PRAK
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C381421
Rabbit Polyclonal (IgG) to Human MAPKAPK5 / PRAK
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Anti-MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C291607
Rabbit Polyclonal (IgG) to Human MAPKAPK5 / PRAK
Human, Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MAPKAPK5 / PRAK
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MAPKAPK5 / PRAK
Goat
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

MAPKAPK5 / PRAK antibody was raised against synthetic peptide C-STEALDNVLPSAQ from an internal region of human MAPKAPK5 / PRAK (NP_003659.2; NP_620777.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Guinea pig, Lizard (100%); Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Medaka, Pufferfish, Zebrafish (92%).
LS-E28033 - Lyophilized - 100 µg - $145.00
aa306-318
Human MAPKAPK5 / PRAK
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11927 or EB11928. Western blots of the IP were labeled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kD of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MAPKAPK5 / PRAK

Q8IW41 NM_003668 NP_003659.2

MAPKAPK5 Antibody, MAPKAP kinase 5 Antibody, MAPKAP-K5 Antibody, MK5 Antibody, p38-regulated/activated kinase Antibody, MAPKAPK-5 Antibody, PRAK Antibody, MK-5 Antibody, p38-regulated protein kinase Antibody

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53.

Immunohistochemistry

Immunohistochemistry
Anti-MAPKAPK5 / PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9567 concentration 5 ug/ml.

Western blot

Western blot
MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with EB11928 (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.

Western blot

Western blot
MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
 

Requested From: United States
Date Requested: 12/9/2016

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