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Catalog Number Size Price
LS-C18843-100 100 µg (0.9 mg/ml) $567 
MAPKAPK2 / MAPKAP Kinase 2 Antibody - Anti-MAPKAP Kinase 2 Polyclonal Antibody - Western Blot. Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kD protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human MAPKAPK2 / MAPKAP Kinase 2 Antibody (IHC, WB) LS‑C18843

Polyclonal Rabbit anti‑Human MAPKAPK2 / MAPKAP Kinase 2 Antibody (IHC, WB) LS‑C18843

Note: This antibody replaces LS-C25409
Antibody:
MAPKAPK2 / MAPKAP Kinase 2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C18843-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAPKAPK2 / MAPKAP Kinase 2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MAPKAP Kinase 2 antibody LS-C18843 is an unconjugated rabbit polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB.
Target
Human MAPKAPK2 / MAPKAP Kinase 2
Synonyms
MAPKAPK2 | MK-2 | MAP KAP Kinase 2 | MAPKAP kinase 2 | MK2 | MAPKAP-K2 | MAPKAPK-2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Specificity
Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
Applications
  • IHC
  • Western blot (1:2000)
  • Immunoprecipitation
  • ELISA (1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody is suitable for western blotting and ELISA.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPKAPK2 / MAPKAP Kinase 2
P49137 NM_004759 NP_004750.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024