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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP3K3 / MEKK3 Antibody (Internal) LS‑C155322
MEKK3 antibody LS-C155322 is an unconjugated rabbit polyclonal antibody to human MEKK3 (MAP3K3). Validated for IHC and WB.
Catalog
Size
Price
LS-C155322-100
100 µl (0.72 mg/ml)
Unavailable

Popular MAP3K3 / MEKK3 Products

Anti-MAP3K3 / MEKK3 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3753 dilution 1:100.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Western blot of MEK Kinase-3 (N96) pAb in extracts from HepG2 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
MAP3K3 / MEKK3 antibody. IHC(P): Rat Brain Tissue.
Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of MEK Kinase-3 antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

MEKK3 antibody LS-C155322 is an unconjugated rabbit polyclonal antibody to human MEKK3 (MAP3K3). Validated for IHC and WB.
About MAP3K3 / MEKK3
MAP3K3 / MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. Q99759 NM_002401 NP_002392.2

MAP3K3 Antibody, MAPK/ERK kinase kinase 3 Antibody, MAPKKK3 Antibody, MEK kinase 3 Antibody, MEKK3 Antibody, MAP/ERK kinase kinase 3 Antibody, MEKK 3 Antibody


Specifications

Target
Human MAP3K3 / MEKK3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MAP3K3 / MEKK3 antibody was raised against recombinant fragment corresponding to a region within amino acids 210 and 488 of MEKK3 (SwissProt Q99759). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (97%).
Epitope
Internal
Specificity
Human MAP3K3 / MEKK3
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/22/2018

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