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Anti-MAP3K3 / MEKK3 Antibody (Internal) LS-C155322

Catalog Size Price
LS-C155322-100 100 µl (0.72 mg/ml) Unavailable

Related Products

26 MAP3K3 / MEKK3 Antibodies

Most Popular MAP3K3 / MEKK3 Antibodies

Anti-MAP3K3 / MEKK3 Antibody IHC-plus™ LS-B3753
Rabbit Polyclonal to Mouse MAP3K3 / MEKK3
Mouse, Human, Rat
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-MAP3K3 / MEKK3 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3753 dilution 1:100.
Anti-MAP3K3 / MEKK3 Antibody (Asn96) LS-C176830
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Human, Mouse
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot of MEK Kinase-3 (N96) pAb in extracts from HepG2 cells.
Anti-MAP3K3 / MEKK3 Antibody (aa228-257) LS-C329805
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
Western blot, ELISA
Unconjugated
Anti-MAP3K3 / MEKK3 Antibody (Internal) LS-C354035
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MAP3K3 / MEKK3 Antibody (aa70-150) LS-C384483
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of MEK Kinase-3 antibody

100% Guaranteed
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Human MAP3K3 / MEKK3
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

MAP3K3 / MEKK3 antibody was raised against recombinant fragment corresponding to a region within amino acids 210 and 488 of MEKK3 (SwissProt Q99759). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (97%).
Internal
Human MAP3K3 / MEKK3
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
For research use only.

About MAP3K3 / MEKK3

Q99759 NM_002401 NP_002392.2

MAP3K3 Antibody, MAPK/ERK kinase kinase 3 Antibody, MAPKKK3 Antibody, MEK kinase 3 Antibody, MEKK3 Antibody, MAP/ERK kinase kinase 3 Antibody, MEKK 3 Antibody

MAP3K3 / MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed.

Immunohistochemistry

IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000

Requested From: 
Date Requested: 4/22/2018

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