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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP3K3 / MEKK3

mitogen-activated protein kinase kinase kinase 3

MAP3K3 / MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: mitogen-activated protein kinase kinase kinase 3
Family/Subfamily: Protein Kinase , MAP3K
Synonyms: MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK3, MAP/ERK kinase kinase 3, MEKK 3
Target Sequences: NM_002401 NP_002392.2 Q99759

Publications (2)

1
Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit nuclear factor kappaB activation. Matitau AE, Scheid MP. The Journal of biological chemistry. 2008 283:13261-8. (IP) [PubMed:18308725]
2
Expression profiles of protein tyrosine kinase genes in human embryonic stem cells. Son MY, Kim J, Han HW, Woo SM, Cho YS, Kang YK, Han YM. Reproduction (Cambridge, England). 2008 136:423-32. (WB) [PubMed:18583472]

Filtered by: None
Target
MAP3K3 / MEKK3 (27)
Reactivity
Human (18)
Mouse (16)
Rat (3)
Bovine (1)
Horse (1)
Monkey (1)
Rabbit (1)
Application
IHC (6)
IHC-P (4)
WB (27)
ELISA (9)
ICC (1)
IF (3)
Peptide-ELISA (1)
Host
rabbit (25)
mouse (2)
Isotype
IgG (19)
IgG2a,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (26)
Clone
1H3 (1)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (2)
Unconjugated (15)
Epitope
aa228-257 (8)
Internal (2)
aa11-25 (2)
70-150 aa, Internal (1)
Asn96 (1)
aa101-150 (1)
aa125-175 (1)
Publications
No (27)
Showing 1-25 of 27 results

MAP3K3 / MEKK3 Antibody IHC-plus™ LS-B3753
Rabbit Polyclonal to Mouse MAP3K3 / MEKK3
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-MAP3K3 / MEKK3 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

MAP3K3 / MEKK3 Antibody (Internal) LS-C354035
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MEKK3 staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MAP3K3 / MEKK3 Antibody (70-150 aa, Internal) LS-C384483
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Mouse, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of MEK Kinase-3 antibody

MAP3K3 / MEKK3 Antibody (Asn96) LS-C176830
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Mouse, Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of MEK Kinase-3 (N96) pAb in extracts from HepG2 cells.

MAP3K3 / MEKK3 Antibody (aa11-25) LS-C313061
Rabbit Polyclonal to Mouse MAP3K3 / MEKK3
Mouse, Rat
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
MAP3K3 / MEKK3 antibody. IHC(P): Rat Brain Tissue.
MAP3K3 / MEKK3 antibody. IHC(P): Rat Brain Tissue.

MAP3K3 / MEKK3 Antibody (Internal) LS-C155322
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
IHC IHC-P WB
Unconjugated
100µl 0.72mg/ml  $355.00
IHC of paraffin-embedded Gastric ca, using MEKK3 antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE MAP3K3 / MEKK3 antibody diluted at 1:1000

MAP3K3 / MEKK3 Antibody (aa228-257) LS-C329805
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
Unconjugated
200µl  $590.00

MAP3K3 / MEKK3 Antibody (aa228-257, Biotin) LS-C262569
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
Biotin Conjugated
200µl  $740.00

MAP3K3 / MEKK3 Antibody (aa228-257, FITC) LS-C262572
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
FITC Conjugated
200µl  $740.00

MAP3K3 / MEKK3 Antibody (aa228-257, APC) LS-C262570
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
APC Conjugated
200µl  $740.00

MAP3K3 / MEKK3 Antibody (aa228-257, PE) LS-C262574
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
PE Conjugated
200µl  $740.00

MAP3K3 / MEKK3 Antibody (aa228-257, AP) LS-C262571
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
AP Conjugated
200µl  $740.00

MAP3K3 / MEKK3 Antibody (aa228-257, Azide-free, HRP) LS-C262573
Rabbit Polyclonal (IgG) to Mouse MAP3K3 / MEKK3
Mouse
ELISA WB
HRP Conjugated, Azide-free
200µl  $740.00

MAP3K3 / MEKK3 Antibody (clone 1H3) LS-C197671
Mouse Monoclonal [clone 1H3] (IgG2a,k) to Human MAP3K3 / MEKK3
Human
ELISA WB
Unconjugated
100µg  $355.00

MAP3K3 / MEKK3 Antibody (aa101-150) LS-C119225
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Mouse, Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from HepG2 cells, using MAP3K3 Antibody. The lane on the right is blocked with the synthesized peptide.

MAP3K3 / MEKK3 Antibody (aa228-257) LS-C164454
Rabbit Polyclonal to Mouse MAP3K3 / MEKK3
Mouse
WB
Unconjugated
400µl  $325.00
Mouse Map3k3 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Map3k3 antibody detected the Map3k3 protein (arrow).

MAP3K3 / MEKK3 Antibody LS-C311381
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
Unconjugated
100µg  $605.00

MAP3K3 / MEKK3 Antibody (Biotin) LS-C523007
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
Biotin Conjugated
100µl  $755.00

MAP3K3 / MEKK3 Antibody (FITC) LS-C533050
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
FITC Conjugated
100µl  $755.00

MAP3K3 / MEKK3 Antibody (AP) LS-C505083
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
AP Conjugated
100µl  $755.00

MAP3K3 / MEKK3 Antibody (APC) LS-C514791
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
APC Conjugated
100µl  $755.00

MAP3K3 / MEKK3 Antibody (aa11-25) LS-C313060
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Human, Monkey
WB
Unconjugated
100µg 200µl 0.5mg/ml  $305.00
WB of MAP3K3 / MEKK3 antibody. Lane 1: HELA Cell Lysate. Lane 2: A431 Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: JURKAT Cell Lysate.

MAP3K3 / MEKK3 Antibody LS-C416107
Rabbit Polyclonal to Human MAP3K3 / MEKK3
Mouse, Human
WB
Unconjugated
100µg  $305.00
Western blot of HepG2 cell lysate using MAP3K3 Antibody

MAP3K3 / MEKK3 Antibody (aa125-175) LS-C289831
Rabbit Polyclonal (IgG) to Human MAP3K3 / MEKK3
Rabbit, Mouse, Bovine, Horse, Human
WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human and Mouse MEKK3 by Western Blot. Samples: Whole cell lysate from 293T (5, 15 and 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MEKK3 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

MAP3K3 / MEKK3 Antibody LS-C309207
Mouse Polyclonal (IgG) to Human MAP3K3 / MEKK3
Human
WB
Unconjugated
50µg  $560.00
Showing 1-25 of 27 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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