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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAD2L1 / MAD2 Antibody (clone 17D10) LS‑C59519
MAD2 antibody LS-C59519 is an unconjugated mouse monoclonal antibody to human MAD2 (MAD2L1). Validated for IP and WB.
Catalog
Size
Price
LS-C59519-100
100 µg
Unavailable

Popular MAD2L1 / MAD2 Products

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Product Description

MAD2 antibody LS-C59519 is an unconjugated mouse monoclonal antibody to human MAD2 (MAD2L1). Validated for IP and WB.
About MAD2L1 / MAD2
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Q13257 NM_002358 NP_002349.1

MAD2L1 Antibody, HSMAD2 Antibody, MAD2 Antibody, MAD2-like protein 1 Antibody


Specifications

Target
Human MAD2L1 / MAD2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal [17D10]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation
Failed Applications
  • Immunofluorescence
Immunogen
MAD2L1 / MAD2 antibody was raised against full-length recombinant human MAD21_1 protein.
Specificity
human MAD21_1 protein. This antibody has only been tested on human cells. Reactivity against homologs from other sources is not known
Usage
This protein A purified antibody has been tested for use in immunoprecipitation and by western blot. This antibody is not applicable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~24 kD corresponding to full- length protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-MAD2L1 Antibody - Western Blot. Western blot of Mab anti-MAD2L1 antibody shows detection of a band at ~24 kD (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.


Requested From: 
Date Requested: 6/19/2018

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