Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human M-phase phosphoprotein Epitope (LTPLK or FTPLQ)
ICC, Western blot, Immunoprecipitation, ELISA
Human M-phase phosphoprotein Epitope (LTPLK or FTPLQ)
Human (tested or 100% immunogen sequence identity)
Protein G purified
Western blot (0.5 - 2 µg/ml)
Specificity and Use
Mitotic human HeLa cell cytosolic lysate.
Recognizes a phosphorylated epitope (S/T) P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type. Species cross-reactivity: animals and plants.
Suitable for use in Western Blot, Immunocytochemistry, ELISA and Immunoprecipitation. Immunoprecipitation: Reported by an independent laboratory. Positive control: Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.
About M-phase phosphoprotein Epitope (LTPLK or FTPLQ)